There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells.

In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45 and a 35kDa isoform, ICAD-S/DFF35. Nuclear chromatin,intracellular,nucleus,nucleoplasm,cytoplasm,lipid particle,cytosol,įunction:Inhibitor of the caspase-activated DNase (DFF40).,PTM:Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 CIDE-N domain.,subunit:Heterodimer of DFFA and DFFB.,įor Research Use Only! Not for diagnostic or therapeutic procedures.ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ,Īpoptosis_Inhibition,Apoptosis_Mitochondrial,Apoptosis_Overview,īreast,Coronary artery,Epithelium,Eye,Kidney,Skeletal muscle, Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFF becomes activated when DFFA is cleaved by caspase-3. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.ĭFFA DFF1 DFF45 H13 DNA fragmentation factor subunit alpha DNA fragmentation factor 45 kDa subunit DFF-45 Inhibitor of CAD ICADĭNA fragmentation factor subunit alpha(DFFA) Homo sapiens Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen ICAD Polyclonal Antibody detects endogenous levels of ICAD protein. The antiserum was produced against synthesized peptide derived from human DFFA.

Icad antibody